TODD-HEWITT BROTH 500 grams/bottle

Todd Hewitt Broth is recommended for the cultivation of streptococci and other fastidious microorganisms. It was originally developed for the production of streptococcal hemolysin. The broth was modified by Updyke and Nickle and is used preferentially to cultivate beta-hemolytic strains, especially for serological typing, from clinical specimens and for epidemiological studies. The medium is also recommended as an enrichment medium for the growth of streptococcal cells in the identification of Groups A and B. This medium was used as an enrichment broth for Group A streptococci in a comparison study of a rapid antigen test. Bacteriological Peptone and Beef Heart infusion provide nitrogen, vitamins, minerals and amino acids essential for growth. Disodium phosphate and Sodium carbonate act as a buffer to prevent the destruction of the hemolysin by the acid produced through fermentation of the carbohydrate Dextrose, source of carbon and energy. Sodium chloride maintains the osmotic balance of medium.