Chromogenic Cronobacter Isolation Agar (CCI) is a selective medium for the detection of Cronobacter spp. in food products and ingredients intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling. ISO 22964:2016 describes a horizontal method for the detection of Cronobacter spp. and recommend this medium for the isolation of Cronobacter spp. Triptone provide nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is source of vitamins, particularly the B-group, essential for bacterial growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Sodium deoxycholate inhibits the accompanying gram positive flora. Sodium thiosulfate increase the selectivity and the recovery of Cronobacter and Enterobacter species. 5-Bromo-4-chloro-3-indolyl a-D-glucopyranoside is the chromogenic substrate. Cronobacter (formerly Enterobacter sakazakii) is currently considered and emerging pathogen responsible for severe meningitis and necrotic enterocolitis in un-weaned babies that can be the cause of mortality rate between 40-80%. The pathogenicity of Cronobacter for un-weaned babies makes it necessary to review the manufacturing process of the milk-based products specialized for babies, guaranteeing the absence of the bacteria in the final product Additional prevention measures at a hospital include the sanitary hygiene of the prepared food; reducing the time between the preparation and its administration, to impede the multiplication of microorganisms.
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