Acetamide Broth contains acetamide which is the sole source of carbon. It is used for the confirmation and identification of Pseudomonas aeruginosa, as specified by the ISO 16266. It uses the ability of non-fermenting Gram-negative bacteria to deaminate the acetamide. The deamination of the acetamide produces ammonia which increases the pH of the medium, acetamide deamination is accomplished by P.aeruginosa, P. acidovorans, Group III (Achromobacter xylosoxidans), and Alcaligenes odorans. Acetamide is the single carbon source. The Potassium salt has a high buffering capacity and Sodium chloride supplies essential electrolytes for transport and osmotic balance. Magnesium sulfate, sodium molybdate and iron sulfate allow the selective growth of Pseudomonas in the medium. It is prepared according to ISO 16266. Pseudomonas aeruginosa is an opportunist pathogen for humans, capable of growing in water with a low concentration of nutrients. This is why natural mineral water and spring water are Pseudomonas aeruginosa free at the time of their commercialization, This mircroorganism can also be found in swimming pool water.
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