WORT BROTH is commonly used for the detection and enumeration of yeasts and fungi, particularly yeasts in butter, syrups and other materials, especially in the soft drinks industry.
Yeasts grow well on media that contain dextrose or maltose, moreover if the reaction is acidic. This medium has been enriched with the addition of salts and other nutrients. The low pH inhibits the growth of most bacteria.
Malt extract and Casein peptone provide nitrogen, vitamins, minerals and amino acids essential for growth. Dextrin is a carbon source. Dipotassium phosphate is the buffer. Glycerol limits bacterial growth by reducing the water activity from 0,999 to 0,95.
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