BPRM BROTH BASE 500 grams/bottle

BPRM Broth Base (Bacteroides Phage Recovery Medium) is a medium recommended by ISO normative 10705-4 for the cultivation of Bacteroides fragilis and for the recuperation of phage from human faecal samples and environmental samples. Bacteroides fragilis is a Gram negative bacteria, an obligate anaerobe and one of the most abundant bacteria in the human colon. It causes 90% of the anaerobic peritoneum infections. This medium allows faster bacterial growth and produces higher phage yields. Peptone and tryptone provide nitrogen, vitamins, amino acids and the nutrients for growth. Glucose is the fermentable carbohydrate, which is a carbon and energy source. Yeast extract is a source of vitamins, particularly of the B-group. Sodium chloride maintains the osmotic balance and magnesium sulfate heptahydrate is the buffering agent in the medium. With the adequate concentration of agar, a semi-solid or a solid medium can be prepared. To inhibit the accompanying flora, add 100 mg/ml of Kanamycin and 7,5 mg/ml of Vancomycin sterilized by filtration.