Lauryl Sulphate Tryptose Broth Modified (mLST) is recommended for the selective enrichment of Cronobacter sakazakii in milk powder and powered infant formula. C. sakazakii is currently considered an emerging pathogen responsible for unweaned babies, risking severe meningitis and necrotic enterocolitis that can be the cause of a mortality rate between 40 and 80%. The pathogenicity of Cronobacter sakazakii for unweaned babies makes it necessary to review the manufacturing process of the products intended for babies, guaranteeing the absence of the bacteria in the final product. Additional prevention measures at hospitals include the sanitary hygiene of the prepared media, reducing the time between the preparation and its administration, to impede the multiplication of microorganisms. Enzymatic digest of plants and animal tissue provide nitrogen, vitamins, minerals and amino acids essential for growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Potassium phosphates act as a buffer system Lactose is the fermentable carbohydrate providing carbon and energy. Sodium lauryl sulfate is the selective agent, inhibiting many organisms except coliforms. The ISO normative 22964 recommended this medium as a selective enrichment with the addition of Vancomycin and incubation at 44ºC. All the tubes must be subcultured in the chromogenic agar for the isolation of Cronobacter sakazakii ESIA (Cat. 1446).
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