Nutrient Broth is used for the general cultivation of a wide variety of microorganisms. The medium supports the growth of a great variety of microorganisms that are not very nutritionally demanding. A feature common to all selective media is that sublethally injured organisms are not generally detected and therefore a recovery step must be included in examination procedures. This is of importance, particularly in the food industry as various processes such as heat, desiccation, preservation processes, pH changes, etc, cause sublethal injuries to microorganisms. The broth is rich in nutrients and produces high resuscitation rates for sublethally injured bacteria and intense growth. This medium is used in accordance with the official recommended procedures for the bacteriological analyses of water, milk, dairy products and feces of clinical samples, and as a base to prepare media supplemented with other nutrients. Nutrient Broth is used in many laboratory procedures as it is or with added indicators, carbohydrates, organic liquids, salts, etc. Bacteriological Peptone and Beef extract provide nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is source of vitamins, particularly the B-group. Sodium chloride supplies essential electrolytes for transport and osmotic balance.
Write Your Own Review