Pseudomonas Agar Base, with CFC Supplement (Cat. 6036) added, is a selective medium recommended by ISO 13720 for the enumeration of Pseudomonas spp in meat and meat products, including poultry. Gelatin peptone and enzymatic digest of casein provide nitrogen, vitamins, minerals and amino acids essential for growth and permits the growth of a great number of Pseudomonas species. The quantities of potassium sulfate and magnesium chloride favor the formation of pigmentation (production of pyocyanin). The addition of CFC Supplement (Cat. 6036) makes the medium more selective for Pseudomonas spp. including Burkholderia cepacia, previously known as Pseudomonas cepacia. Cetrimide, fucidin and cephaloridine inhibit Gram-positive bacteria and support the growth of Pseudomonas spp, (including P. aeruginosa), whilst inhibiting most other Gram-negative bacteria. The colonies of Pseudomonas spp. presuntives are confirmed by the oxidase test (positive). - The colonies change to violet color after 5-10 s. Positive oxidase. - There is no change of color. Negative oxidase.
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