EVA Broth (Ethyl, Violet, Azide, Litsky) is a selective medium specific for the detection and confirmation of enterococci in water, other specimens and foods. It is an indication of fecal contamination. EVA Broth, in conjunction with Rothe Broth (Glucose Broth with Azide - Cat. 1238) as the presumptive medium, is used to enumerate fecal enterococci in water, soil and food products by the MPN technique. The presence of intestinal enterococci is an indicator for faecal contamination, especially when the contamination occurred long ago and the less resistant coliform bacteria, including Escherichia coli, are already dead when the analysis is carried out. Sodium azide and Ethyl violet inhibit all Gram-positive bacilli and Gram positive cocci except enterococci and Gram negative bacteria. Peptone mixture provides nitrogen, vitamins, minerals and amino acids essential for growth. Dextrose is the fermentable carbohydrate providing carbon and energy. Potassium phosphates act as a buffer system, and Sodium chloride supplies essential electrolytes for transport and osmotic balance.
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