Giolitti-Cantoni Broth with Potassium tellurite added is prepared according to the formula of Giolitti and Cantoni, for the enumeration, using the MPN Method, and for the selective enrichment of coagulase positive staphylococci in foodstuffs and dried baby milk. It was designed by Giolitti and Cantoni to facilitate the growth of Staphylococcus aureus by incorporating Sodium pyruvate in the formula, even when present in low numbers in food samples. The International Dairy Federation recommends this medium in a procedure for detecting S. aureus in dairy products, using it as an enrichment medium. The Tryptone and Beef extract provide nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is a source of vitamins, particularly of the B-group essential for bacterial growth. Mannitol is the fermentable carbohydrate providing carbon and energy. The growth of Gram-negative bacteria is inhibited by Lithium chloride, whilst the Gram-positive bacilli are inhibited by Glycine and the Potassium tellurite. The high level of Potassium tellurite is necessary to suppress the high numbers of contaminating organisms that could be expected.
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