Listeria Selective Enrichment Broth, Modified (UVM Formula) is a modification of the formula described by Donnelly and Baigent; reducing the content of Nalidixic Acid in UVM-I and UVM-II from the previous formulation and increasing the concentration of acriflavine hydrochloride in the UVM-II Listeria Selective Enrichment Broth, Modified Cat. 1280. This modification and the two-stage selective enrichment method (USDA-FSIS) have meant the most isolation of Listeria monocytogenes in meat products and, additionally, have the advantage of doing so in only 3-4 days. Tryptone, proteose peptone and beef extract provide nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is a source of vitamins, particularly of the B-group essential for bacterial growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Potassium phosphates act as a buffer system. Esculin is hydrolyzed by all Listeria species. Nalidixic acid blocks the DNA replication of susceptible bacteria and acts against many Gram-negative bacteria. Acriflavine hydrochloride inhibits many Gram-positive bacteria.
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