TRYPTICASEIN SOY AGAR (T.S.A.) Nº2 500 grams/bottle

Trypticasein Soy Agar (TSA)  Nº2 is an improved formulation of the original TSA Agar, to be used with animal blood supplements with 5 or 10% sheep blood. It is used for the isolation, cultivation and detection of hemolytic reactions of fastidious microorganisms. These hemolytic reactions are important to differentiate bacteria from clinical samples, especially species of Streptococcus. Trypticasein Soy Agar  Nº2, supplemented with 5% sheep blood, offers clear and visible hemolytic reactions with group A streptococci (Streptococcus pyogenes). Tryptone H enhances hemolysin production. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Soy peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Bacteriological agar is the solidifying agent. Blood is an additional source that provides growth factors for the microorganisms and is the basis for determining haemolytic reactions. Hemolytic streptococci can be seen as translucent or opaque, grayish, small (1 mm), or large matt and mucoid (2-4 mm) colonies, surrounded by a hemolysis zone. Staphylococci appear as opaque, white to gold-yellow colonies with or without zones of beta hemolysis.