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Enterococcus Confirmatory Agar is used to confirm the presence of enterococci in water and other sources of sanitary interest. The presence of intestinal enterococci, also known as fecal streptococci, is an indicator for fecal contamination, especially when the contamination occurred a long time ago and the less resistant coliform bacteria, including Escherichia coli, may already be dead at the time of analysis. Casein peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is a source of vitamins, particularly of the B-group essential for bacterial growth. Dextrose is the fermentable carbohydrate providing carbon and energy. Sodium azide inhibits Gram negative bacteria and Methylene blue is an inhibitor of some Gram positive bacteria. Methylene blue is also the pH indicator. Bacteriological agar is the solidifying agent. To the prepared test tubes, in order to cover half of the slanted surface, aseptically add a volume of either Enterococcus Selective Broth (Cat.1204) or Enterococcus Confirmatory Broth ( same formulation as this medium but without the agar). Using growth from KAA Presumptive Broth (Cat. 1209), inoculate both the surface and the broth in the ConfirmatoryAgar/Broth mixture tube.
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