WL Nutrient Broth, based on the Green and Grey formulation, is recommended for the control of industrial fermentations, particularly the manufacturing of beer. With a pH of 5,5 true counts of beer yeasts can be made. With a pH of 6,5 the medium is ideal for bakery and distilled spirit yeasts. Tryptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Dextrose is the fermentable carbohydrate providing carbon and energy. Yeast extract is a source of vitamins, particularly of the B-group. Monopotassium phosphate is the buffer. Potassium, Calcium and Ferric chlorides all provide essential ions for the osmotic balance. Manganese and magnesium sulfate are a source of divalent cations. Bromocresol green is the pH indicator.
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