EC Medium was developed by Hajna and Perry, for the selective identification of coliform bacteria and Escherichia coli in water, foodstuffs and other materials. It is recommended by ISO 7251, for the enumeration of E.coli with MPN tecnique. This medium improves the detection methods of the coliform group, in particular of E.coli, and is used to investigate drinking water, wastewater treatment systems and generally for water-quality monitoring, as well as shellfish and other foods. It is used in many Standard Methods for Food and Water testing. The medium can be used at 35Â±2 °C for detection of coliform organisms or at 44,5 °C for isolation of E. coli. The bile salts act as selective agent inhibiting Gram-positive bacteria, bacilli and enterococci but allowing E. coli to develop. The potassium salts have a high buffering capacity. Enzymatic digest of casein provides the nutrients for growth and lactose is the fermentable carbohydrate as carbon and energy source. Sodium chloride maintains the osmotic balance.
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