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Rose Bengal Agar + Chloramphenicol is a neutral selective medium recommended for the enumeration of molds and yeasts in foods, water and environmental materials. Rose-Bengal Chloramphenicol Agar is recommended for fresh proteinaceous foods with flora mostly made up of Gram-negative rod-shaped bacteria. It is also appropriate when higher and longer incubation temperatures, around 35°C, are required. Bacteriological peptone provides the nitrogen, vitamins, minerals and amino acids source. Dextrose is the fermentable carbohydrate as a carbon and energy source. Potassium phosphate is the buffer. Magnesium sulfate provides sulfur and other trace elements. Rose bengal is a selective agent that inhibits the growth of bacteria and limits the size and height of faster-growing molds, allowing for the development and detection of other slower-growing yeasts - molds appear pink colored. Chloramphenicol serves as a selective agent, inhibiting bacterial growth. It is a recommended antibiotic for neutral media due to its heat stability and wide bacterial spectrum. Bacteriological agar is the solidifying agent.
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