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ESBL (Extended Spectrum ß-Lactamases)is a Chromogenic medium for the detection of gram-negative bacteria producing Extended Spectrum Beta-Lactamase. ESBL (Extended Spectrum ß-Lactamases) are enzymes capable of hydrolyzing penicillins, broad-spectrum cephalosporins and monobactams, ESBLs are often located on plasmids that are transferable from strain to strain and between bacterial species. ESBL-producing Enterobacteriae were first identified in Germany in 1983, and now they are widely recognized as clinically relevant causes of infections in community. During the 1990s were mostly found in Klebsiella species. However E. coli ESBL-producing has also been widely detected and both have a significant importance in hospital acquired infections. Community-acquired urinary tract infection (CA-UTI) is the most common infection caused by extended-spectrum ß-lactamase (ESBL)-producing Enterobacteriaceae and it is a big concern in management of patients and hospital costs. The development and spread of ESBL among Gram-negative bacteria and possible horizontal transfer calls for concern, especially in view of treatment failure, high treatment cost, and consequent discomfort to patients. The early detection of ESBL-producing bacteria carriers is essential to minimize their impact and spread. Peptones and growth factors provide nitrogen, vitamins, minerals and aminoacids essential for growth. Chromogenic mixture allows the identification of ESBL producing microorganisms. The supplement inhibits the growth of all the non ESBL-producing bacteria. Characteristics of the ESBL colonies: - E. coli: pink colonies. - Enterobacter aerogenes: dark blue colonies. - Klebsiella pneumoniae: dark blue colonies.
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