Esculin Iron Agar is a medium for the confirmation of enerococcal colonies on membrane filters through which water samples have been filtered and which have been incubated on a appropriate medium for enterococcus. The membrane is transferred to the Esculin Iron Agar and incubated at 41 ºC during 20 minutes. The colonies turn into a black color due to the esculin hydrolyzation to esculetin, which reacts with iron ions producing a blackening of the medium. Bacteriological agar is the solidifying agent.
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