Desoxycholate Agar is a selective and differential medium for the isolation and differentiation of Gram negative enteric bacilli. Leifson demonstrated improved recovery of intestinal pathogens from specimens containing normal intestinal flora. The Desoxycholate and Citrate salts inhibit the development of the Gram positive organisms. The Peptone mixture provides nitrogen, vitamins, minerals and amino acids essential for growth. Lactose is the fermentable carbohydrate providing carbon and energy. Dipotassium phosphate acts as a buffer system. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Neutral red is a pH indicator. Bacteriological agar is the solidifying agent.
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