Stuart Transport Medium is a semi-solid medium used for the transport and preservation of biological samples when immediate inoculation into a culture medium is not possible. It is a suitable medium for the cultivation of various organisms such as gonococci, streptococci, enterobacteria, etc. It was described by Stuart in 1946, and later, it was used to preserve the viability of fastidious microorganisms such as Neisseria spp. or Haemophilus influenzae. All specimens should be transported to the laboratory as soon as possible and maintained at room temperature since chilling may be detrimental to some organisms. Stuart Transport Medium is essentially non-nutritive; it contains sodium thhioglycollate, which helps delay oxidation and allows a better recovery of anaerobics. Calcium chloride, together with sodium glycerophosphate, act as a buffering agent that maintains the osmotic balance of the medium. Methylene blue is the redox indicator; the blue color indicates the presence of oxygen. Despite the sodium thioglycolte, the medium can undergo a slight oxidation in the upper part of the tube, which acquires a blue coloration. If a distinctive blue color is observed, discard the tube.
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