Buffered Listeria Enrichment Broth with Supplements (BLEB Broth) is a medium used for the selective enrichment of Listeria spp from food and environmental samples. BLEB Broth is a modification of the formula by Lovett et al. with more concentration of disodium phosphate in order to increase the buffer capacity and improve the growth of Listeria species. Tryptone and soy peptone provide nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is the source of vitamins, particularly of the B-group. Potassium phosphates act as a buffer system. Dextrose is the fermentable carbohydrate providing carbon and energy. Sodium chloride supplies essential electrolytes for osmotic balance. Nalidixic acid, acryflavine and cycloheximide inhibit the growth of the accompanying flora.
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