KING B MEDIUM (PSEUDOMONAS F AGAR) (USP) 500 grams/bottle

King B Medium (Pseudomonas F Agar) is prepared according to the formula described by King et al. for the detection and differentiation of Pseudomonas aeruginosa (among other Pseudomonas), based on pyocyanin production. The medium is recommended by ISO 16266 for the confirmation of reddish brown colonies in the Pseudomonas CN Agar (Cat. 1153) that have been positive oxidase. This method is recommended for bottled water, or for other types of water that have little background interfering flora, such as pool waters and water intended for human consumption. Pseudomonas aeruginosa is a free-living bacterium, present in soil and water. It has become more and more known as an emerging opportunistic pathogen of clinical importance. Various different epidemiological studies track its occurrence as a nosocomial pathogen and claim that antibiotic resistance is increasing in clinical isolates. Peptone mixture provides nitrogen, vitamins, minerals and amino acids essential for growth. It also promotes the production of pyocyanin. Dipotassium phosphate is a phosphorus source, and magnesium sulfate provides cations to activate pyocyanin production. Glycerol is a carbon source. Bacteriological agar is the solidifying agent.