Phenylalanine Agar is a solid medium used for differentiating Proteus, Providencia and Morganella species from other Enterobacteriaceae, based on the deamination of phenylalanine to phenylpyruvic acid by enzymatic activity. The formula is prepared according to Ewing et al. (1957). Some strains of Enterobacter and a few non-fermenting Gram-negative bacilli are also capable of deaminating phenylalanine. DL-Phenylalanine is deaminated to phenylpyruvic acid. Yeast extract provides vitamins, particularly of the B-group, and other nutrients for growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Sodium phosphate is the buffer and Bacteriological agar is the solidifying agent.
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