RM BASE MEDIUM 500 grams/bottle

RM Base Medium is used for the maintenance and propagation of the promoter PL in E. coli strains GI724, GI826 and GI698 and to increase the yield of plasmid for sequencing positive clones. These strains have the gene Lambda cI repressor, under the control of the promoter tryptophane inducible, trp. This medium has low tryptophane levels. Casaminoacids provides the necessary nutrients and cofactors required for excellent growth of recombinant strains of E. coli. Due to its higher degree of digestion, casaminoacids are an excellent source of free aminoacids. Phosphates act as a buffer system. Ammonium chloride and magnesium sulfate provide essential ions. Sodium chloride supplies essential electrolytes for transport and osmotic balance. To promote growth it may require the addition of glucose.