SIM MEDIUM 500 grams/bottle

SIM Medium is a semisolid medium used for the differentiation of enteric bacteria through sulfide production, indole formation and motility.The sulfur reduction test is useful in differentiating enteric organisms, especially Salmonella and Shigella. The indole test is used for differentiating the Enterobacteriaceae. The motility test is useful for testing a wide variety of organisms. The medium is also useful in the differentiation of Klebsiella from Enterobacter and Serratia species. Casein and Meat peptones provide nitrogen, vitamins, minerals and amino acids essential for growth. Casein peptone is rich in tryptophan which is reduced and produces indole. Sodium thiosulfate provides Sulphur and Ferric ammonium citrate is the indicator for H2S production under alkaline conditions. Bacteriological agar is the solidifying agent in a low-concentration to enable the motility to be seen.