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Wilkins Chalgren Medium II is used in studies of antimicrobial susceptibilities with both the broth and the agar, standardizing by using identical nutrient formulation media. This medium is also recommended to grow anaerobic microorganisms. It has the advantage over other media in that it does not need the addition of blood to obtain the satisfactory growth of clinically important anaerobic bacteria. Yeast extract provides vitamins, particularly the B-group, and other growing factors to cultivate Bacteroides melaninogenicus and Peptostreptococcus anaerobius. Tryptone and peptone provide nitrogen, vitamins, minerals and amino acids essential for growth. Dextrose is the carbohydrate energy source. L-Arginine provides amino acids for the growth of Eubacterium lentum. Sodium pyruvate acts as an energy source for saccharolytic cocci, such as Veillonella, and to catalyze and degrade traces of hydrogen peroxide which affects the metabolism of anaerobes. Hemin is essential for the growth of Bacteroides spp. Sodium chloride supplies essential electrolytes for transport and osmotic balance.
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