NZCYM Broth is used as an enrichment medium for the cultivation of recombinant strains of E.coli and propagating lambda bacteriophages developed by Blattner and colleagues. E.coli grows fast in this enrichment medium, which supplies the amino acids, vitamins and other metabolites as nucleotide precursors and other factors that otherwise would be synthesized by the cell. Casein digest, Yeast extract and Casamino acids provide the necessary nutrients and cofactors required for excellent growth of recombinant strains of E.coli. Magnesium sulfate is the magnesium ions font required in a big variation of enzymatic reactions, including DNA replication.
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