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Iso-Sensitest Agar 500 grams
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Iso-Sensitest Agar is used as a semi defined medium designed for antimicrobial susceptibility test. This media was developed under a specific formulation to create a controlled formula with stability in mineral content and avoid the undefined components in order to perfectly control and allow reproducible assays. This media allows the growth of the great majority of microorganisms without supplementation. With this media it is avoided the main objections against Mueller Hinton Formula, both in broth and agar: - Agar versions showing antagonistic effects towards tetracycline. - High levels of sulphonamide and trimethoprim antagonists - Poor growth-supporting ability for streptococci and variable growth rates with gram-positive organisms generally. Bacteriological agar is the solidifying agent. Dextrose is the fermentable carbohydrate providing carbon and energy. Hydrolyzed peptone and peptone casein provide nitrogen, vitamins, minerals and amino acids essential for growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Magnesium sulphate, calcium gluconate, cobaltous sulphate, cupric sulphate, zinc sulphate, ferrous sulphate, manganous chloride are ions required in a big variation of enzymatic reactions. Menadione is a synthetic chemical compound sometimes used as a nutritional supplement because of its vitamin K. Cyanocobalamin is a source of B12. L-Cysteine hydrochloride is the reducing agent. Biotin, also known as vitamin H or coenzyme R is a water-soluble B-vitamin (vitamin B7). Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Pyridoxine is one of the compounds that can be called vitamin B6. Pantothenate is the source of B5. Nicotamide is the source of B3. Thiamine is the source of vitamin B1. Tryptophan, Adenine, Guanine, xanthine and uracil are the source of aminoacids.
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