Urea Indole Broth can be used for the determination of urease and indole production by Enterobacteriaceae as well as microorganisms of the families of Brucella, Bacillus, Micrococcus, Mycobacteria and Proteus. L-Tryptophan is an essential amino acid and is converted to skatole and indole. Sodium chloride maintains the osmotic balance. Potassium phosphates act as a buffer system. Urea is a source of nitrogen for those organisms producing urease. Phenol red is the pH indicator. This medium is recommended for the presumptive identification of Yersinia enterocolitica.
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