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Actinomycete Isolation Agar with Glycerol is used for the isolation and cultivation of actinomycetes from soil and water. Actinomycetes are a group of bacteria with many features. They produce antibiotics and other therapeutically useful compounds and are very important in the cycling of organic matter in the soil ecosystem. They are also found in water and vegetation. The isolation of actinomycetes from the mixed microflora present in nature is complicated by their characteristic slow growth relative to that of other soil bacteria. Sodium propionate is a substrate used in anaerobic fermentation. Sodium caseinate is used as source of nitrogen. Dipotassium phosphate is used as a buffer. Asparagine is an amino acid that provides organic nitrogen. Magnesium sulfate and Ferrous sulfate are ions required in a big variation of enzymatic reactions, including DNA replication. Bacteriological agar is the solidifying agent.
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