Columbia Broth, being highly nutritive, is used for the cultivation of microorganisms in general, as even the most fastidious grow in it. This medium is prepared according to the formulation described by Morello and Ellner in their study of Columbia Broth. A medium developed for blood cultures, it is superior to commonly used general since it allows a faster growth of Staphylococcus aureus, E. coli and streptococci (Viridans and Enterococcus). Columbia Broth, in the presence of CO2 and supplemented with the anticoagulant SPS (Sodium polyanetholsulfonate), is an excellent blood culture medium. Peptone mixture and tryptic digest of beef heart provide nitrogen, vitamins, minerals and amino acids essential for growth. Dextrose is the fermentable carbohydrate providing carbon and energy. L-Cysteine HCl is the reducing agent. The medium is buffered with TRIS Aminomethane and TRIS Aminomethane HCl. Ferrous sulfate is added to facilitate organism growth. Magnesium sulfate is a magnesium ion required in a large variation of enzymatic reactions. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Sodium carbonate is a neutralizer that will absorb any toxic metabolites.
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