Rogosa SL Broth is used for the isolation, enumeration and identification of lactobacilli in oral bacteriology, saliva, feces, vaginal specimens and foodstuffs. Rogosa SL Broth is a modification of media described by Rogosa, Mitchell and Wiseman. Rogosa SL Broth is similar to Rogosa SL Agar (Cat. 1096), but lacks the agar and is very selective due to its high sodium acetate and ammonium citrate concentrations and its low pH, which is very advantageous for the cultivation of lactobacilli and inhibits most microorganisms including streptococci and molds and limits swarming but allows the growth of lactobacilli. Sucrose, arabinose and dextrose are fermentable carbohydrates as carbon and energy sources. Tryptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is a source of vitamins, particularly of the B-group. Sulfate salts provide inorganic ions; Sorbitan monooleate is a surfactant and is incorporated to neutralize phenols, hexachlorophene and formalin. Monopotassium phosphate acts as a buffer system.
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