APT Agar (All Purpose Tween) 500 grams/bottle

APT Agar was formulated by Deibel, Evans and Niven when they were investigating thiamine requiring bacteria and heterofermentative lactobacilli. Lactobacillus forms a major part of lactic acid bacteria group which are abundant in nature. They convert lactose and other sugars to lactic acid and therefore are named as Lactobacillus. This media is recommended for the microbiological examination of canned meat, poultry, sauerkraut and other food. APT Agar is also used in the microbiological assay of thiamine. APT Agar contains peptone mixture, which acts as a source of carbon, nitrogen, vitamins and minerals. Yeast extract provides vitamin and B-complex nutrients, which is required for the growth of bacteria. Dextrose is the carbohydrate source. Manganese chloride, magnesium sulphate and ferrous sulphate are essential for the replication of lactobacilli and lactic acid streptococci. Polysorbate 80 is a source of fatty acids required by lactobacilli. Sodium citrate partially inhibits the growth of Gram negative bacteria.