Lauryl Sulfate Agar is a selective medium used in the presumptive detection of coliforms in waters, dairy products, seafood and foods, according to APHA Standard Methods, and by using the membrane filtration technique. The coliform group is both aerobic and anaerobic facultative, Gram-negative, non-spore forming rods which ferment lactose producing acid and gas at 35ºC within 48 hours. Casein peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is the source of vitamins, particularly of the B-group. Lactose is a fermentable complex carbohydrate energy source. Sodium lauryl sulfate is the selective agent used to inhibit organisms other than coliforms. Sporulating aerobic bacteria are completely inhibited. Phenol red is a pH indicator. Bacteriological agar is the solidifying agent.
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