INDOLE NITRATE MEDIUM 500 grams/bottle

Indole Nitrite Medium (Trypticasein Nitrate Medium) is a semisolid medium used to determine nitrate reduction and indole production by a wide variety of organisms. The reduction of nitrate is an important biochemical tool in the identification of many microorganisms. Nitrate reduction is an important characteristic of most members of the Enterobacteriaceae family. Casein peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Potassium nitrate acts as the substrate for determining nitrate reduction. Dextrose is the fermentable carbohydrate providing carbon and energy. This medium is used to identify Gram-negative bacilli using 2 tests: one for Indole production and another for Nitrate reduction. Indole Nitrite Medium can be used for nitrite tests with members of the Enterobacteriaceae family but is not recommended for the indole test with these organisms since they reduce nitrate to nitrite, thus preventing indole detection.