Tryptose Dextrose Agar with thiamine is a general purpose non selective medium recommended for the cultivation of Brucella spp. Sanders and Huddleson demonstrated that the addition of dextrose and thiamine chlorohydrate to the medium stimulates the growth of some species of Brucella. The Tryptose Dextrose Agar with thiamine is also recommended for the cultivation of pathogen microorganisms without enrichment, for streptococci, pneumococci, meningococci and other fastidious bacteria. Tryptose provides nitrogen, vitamins, minerals and amino acids essential for growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Dextrose is the fermentable carbohydrate providing carbon and energy. Thiamine is a growth factor. Bacteriological agar is the solidifying agent. The high productivity of the Tryptose Dextrose Agar used for the cultivation and isolation of Brucella confirms its value for the primary cultivation of Brucella, as other fastidious organisms.
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