Elliker Medium, also known as Lactobacilli Broth, is a medium recommended for the general cultivation of streptococci and lactobacilli. Testing dairy products for lactic acid bacteria facilitates the determination of acid levels, evaluation of lactic starter cultures and helps to control the quality of cured cheese, cultured milks and uncultured products. The medium is prepared according to the formula of Elliker, which has a slightly acidic pH and contains sufficient nutrients to support the growth of these gram-positive microorganisms. Gelatin, tryptone and yeast extract provide the essential nutrients for growth. Lactose, sucrose and dextrose are the fermentable carbohydrates providing carbon and energy. Ascorbic acid provides adequate acid conditions. Sodium chloride supplies essential electrolytes for transport and osmotic balance and sodium acetate is the selective agent inhibiting gram-negative bacteria and also acts as a buffer system.
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