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Fecal Coliforms Broth Base (m-FC) is prepared according to the formula proposed by Geldreich, Clark and Bert and it is used for the cultivation and enumeration of fecal coliforms microorganisms. This medium is suitable for the membrane filter technique at a high temperature. Many standard procedures specify the use of Fecal coliforms Media for testing water and foods. Fecal coliforms are differentiated from other coliforms from environmental sources by their ability to grow at 44,5±0,5 ºC. Proteose and Tryptose provide nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is a source of vitamins, particularly of the B-group essential for bacterial growth. Lactose is the fermentable carbohydrate as a carbon and energy source. Bile salts and aniline blue inhibit growth of Gram-positive bacteria. Sodium chloride maintains the osmotic balance. Aniline blue and rosolic acid, which is provided by the supplement, are the differential indicators. Bacteriological agar is the solidifying agent.
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