Brucella Medium Base is prepared according to the formula described by Jones and Brinley Morgan for the cultivation and isolation of Brucella, including fastidious types. It is a medium rich in nutritive elements and growth factors that make it adequate for the growth and isolation of Brucella spp. Beef extract and peptone provide nitrogen, vitamins, minerals and amino acids essential for growth. Glucose is the fermentable carbohydrate providing carbon and energy. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Bacteriological agar is the solidifying agent. The addition of the supplement enhances the mediums to selectivity for the growth of Brucella. Brucella species are level 3 pathogens and cause brucellosis, a zoonotic disease. It is usually transmitted through milk, dairy products, meat and direct contact with infected animals. It is widely used for the isolation of Brucella in highly contaminated materials, food materials and clinical samples.
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