Lowenstein Jensen Medium Base w/o M.Green 500 grams/bottle

Lowenstein Jensen Medium Base w/o M.Green can be used, with whole egg, glycerol and malachite green, to cultivate and isolate a wide variety of mycobacteria other than M. leprae from clinical samples. The growth of mycobacteria on egg media can be used for niacin testing. Glycerol and egg mixture provide fatty acids and protein necessary for the metabolism of mycobacteria. The coagulation of the egg albumin during sterilization gives a solid medium for inoculation purposes. Monopotassium phosphate acts as a buffer system. Magnesium sulfate is a magnesium ion required in a large variation of enzymatic reactions, including DNA replication. Malachite green suppresses the growth of contaminating bacteria. With 5% sodium chloride, Lowenstein Jensen Medium can be used as an aid in the differentiation of rapid-growing mycobacteria from slow growers on the basis of salt tolerance. M. fortuitum, M. triviale, M. chelonei and some strains of M. flavescens grow on this medium while most other mycobacteria strains are inhibited. Lowenstein Jensen Medium in a deep-butt tube may be used to aid the differentiation of mycobacteria on the basis of the catalase test. Lowenstein Jensen Medium with antibiotics can be used to selectively isolate mycobacteria and inhibit contaminating flora. The addition of ribonucleic acid to the Lowenstein Jensen Medium may increase percentage of tubercle bacilli recovered from clinical specimens compared to recovery on the standard Lowenstein Jensen Medium. M. bovis will not grow on Lowenstein Jensen Medium containing glycerol.