Bolton Selective Enrichment Broth is used for the selective preenrichment of Campylobacter in food samples with low numbers of campylobacters and low level of background microflora and/or with stressed campylobacters. It is recommended by ISO 10272. Campylobacter genus are gram-negative, microaerophilic bacteria that can be present in milk, none treated water or undercooked food. Injured organisms are not generally detected and therefore a recovery step must be included in examination procedures. This is of importance, particularly in the food industry as various processes such as heat, desiccation, preservation processes, pH changes, etc, cause sublethal injuries to Campylobacter. The broth is rich in nutrients and produces high resuscitation rates for sublethally injured bacteria and intense growth. Meat Peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Lactalbumin Hydrolysate provides nitrogen, amino acids, vitamins, and carbon and is specially indicated for culture media for Campylobacter growth. Yeast extract is source of vitamins, particularly the B-group. Sodium chloride supplies essential electrolytes for transport. Alpha-ketoglutarate acid is incorporated to satisfy the specific nutritional requirements of Campylobacter species. Sodium pyruvate is a source of energy for bacterial metabolism and aids in resuscitation of stressed organisms. Sodium carbonate is a pH regulator. Hemin provides X factor, which stimulates the growth of many microorganisms. The Addition of Bolton Broth Selective Supplement inhibits the accompanying gram-positive bacteria due to Trimetropin, gram-negative bacteria due to the Vancomycin, Cephoperazone and Trimetropin as well as yeasts and moulds due to Cycloheximide.
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