Modified Semisolid Rappaport Vassiliadis Medium (MSRV) is a selective medium used for the rapid detection of motile Salmonella spp. Is a modification of Rappaport Vassiliadis enrichment broth for detecting motile Salmonella spp in feces, food products and environmental samples. In this medium the main detection is based on the motility and ability of Salmonella to migrate through selective medium ahead of competing motile microorganism, therefore producing opaque halos of growth. The mobility of other microorganisms is inhibited by selective mediums (such as magnesium chloride, malachite green oxalate and novobiocin) as well as by the temperature of incubation at 42 °C. Tryptose and acid casein peptone provide nitrogen, vitamins, minerals and amino acids essential for growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Magnesium chloride and malachite green oxalate are inhibitory to organisms other than Salmonella spp. Novobiocin is a selective agent that inhibits gram positive bacteria and avoids the development of Proteus. This medium is not suitable for the detection of non motile strains of Salmonella whose of which their presence is very low (= 1 %). It is recommended to conduct serological and biochemical tests for Salmonella species confirmation.
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