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SKU
GE/17040401
Brand
CYTIVA (LIFESCIENCE)
Pre-order (Deliver in 8 to 12 weeks)

HITRAP PROTEIN G HP, 5 X 1 ML (STORE IN CHILLER : 2 TO 8 DEG C)

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HiTrap Protein G HP are prepacked columns for general-purpose preparative purification of monoclonal and polyclonal antibodies from most species including rat.

  • First choice Protein G resin for routine purification of antibodies: ensures minimized sample dilution and high resolution.
  • The small bead size (34 µm) ensures narrow elution peaks containing concentrated material.
  • High protein recovery due to high binding capacity of 25 mg IgG/mL resin.
  • Broader selectivity compared with protein A ligand.
  • Convenient HiTrap format for easy connection to a syringe, peristaltic pump, or chromatography system such as ÄKTA system.

Protein G column for general-purpose antibody purification

HiTrap Protein G HP columns are prepacked with Protein G Sepharose High Performance resin. This protein G affinity resin is a good first choice for general-purpose capture of antibodies at laboratory scale, because it binds to a broader range of IgG from eukaryotic species and also binds to more subclasses of IgG than protein A-based resins do.
Use these columns for IgG purification from cow, sheep, horse, rat, mouse, and human sources, including ascites, serum, and cell culture supernatants. HiTrap Protein G HP columns deliver high-performance, high-resolution purifications with a binding capacity of 25 mg IgG/mL resin and fast kinetics. Columns are stable over a wide pH range – operational pH 3 to 9, and pH 2 to 10 for cleaning.

Successful use in published research

Protein G HiTrap columns are relied upon for a variety of research applications, including:

Other formats of Protein G Sepharose HP resin

Protein G Sepharose High Performance resin is also available in the following formats:
  • Protein G HP SpinTrap spin columns, for small sample purification using a microcentrifuge
  • Protein G HP MultiTrap 96-well plates, for high-throughput screening and small-scale purification using centrifuge or vacuum equipment
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Brand CYTIVA (LIFESCIENCE)
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