Aeromonas Agar Base (RYAN) is based on the formulation of Ryan. It is a modification of the XLD Medium that has been designed to improve the count and isolation of Aeromonas in clinical and environmental samples. The medium is better than other media in the detection of Aeromonas in water, bottled water and food (meat, fish, etc). Proteose peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. L-Lysine and L-Arginine provide nitrogen, sulfur and trace elements. Yeast extract is a source of vitamins, particularly the B-group. Inositol, lactose, sorbitol, and xylose are the carbohydrate substrates. Sodium thiosulfate provides sulphur, and ferric ammonium citrate is the indicator for H2S production. H2S positive colonies have a black center. The mixed indicators bromothymol blue and thymol blue change their color to yellow when acid is formed. Sodium chloride supplies essential electrolytes for transport and osmotic balance and Bile Salts are inhibitors of Gram-positive organisms. Aeromonas are aquatic organisms, fresh water bacteria that were identified during the last century as responsible for infectious processes in aquatic animals: amphibians, reptilian, fish, snails, and others. Aeromonas also cause intestinal tract diseases. Infections are contracted frequently due to direct exposure to the water in which these microorganisms live. This medium is also used for clinical diagnoses.
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