Induction Base Medium is used for the maintenance and propagation of the PL promoter in E. coli strains GI724, GI826 and GI698 and to increase plasmid yield during sequencing of positive clones. These strains contain the Lambda cI repressor gene under the control of the tryptophan-inducible trp promoter. This medium has low levels of tryptophan. Casaminoacids provide the necessary nutrients and cofactors for a good growth of recombinant E. coli strains. Due to their greater degree of digestion, casaminoacids are an excellent source of free amino acids. Phosphates act as a buffer system. Ammonium chloride and magnesium sulfate provide essential ions for transport and osmotic balance.
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