Orange Serum Agar is recommended for the isolation, cultivation and enumeration of acid-tolerant spoilage microorganisms in fruit juice and fruit juice concentrates, in particular from citrus fruit. The low pH of fruit juices makes citrus fruit products susceptible to spoilage by yeasts, molds and the bacteria Lactobacillus and Leuconostoc. Hays investigated spoilage in frozen concentrated orange juice. He found that an agar medium containing orange serum was superior to others in isolating the microorganisms responsible for spoilage. Orange Serum Agar is specially indicated for growing the lactic acid micro flora that spoil citric products, such as Lactobacillus, Leuconostoc and molds. Casein peptone is source of nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is source of vitamins, particularly the B-group essential. Glucose is the fermentable carbohydrate providing carbon and energy. Monopotassium phosphate acts as a buffer. Bacteriological agar is the solidifying agent. The low pH of citric juices and products limits the growth of microorganisms to acid tolerant pathogens.
Write Your Own Review