Muller-Kauffmann Broth Base with Brilliant Green and Novobiocin (MKTTN) is recommended by the ISO 6579 and ISO 19250 norms to be used as a selective enrichment broth for the detection of Salmonella spp in all food types, including milk and dairy products, molluscan shellfish and other fish products, and in water samples and environmental swabs. Beef extract and casein peptone provide nitrogen, vitamins, minerals and amino acids essential for growth. Calcium carbonate is a neutralizer which absorbs toxic metabolites. Bile salts, brilliant green and novobiocin inhibit organisms other than Salmonella. Selectivity is also obtained by both sodium thiosulfate and tetrathionate, suppressing coliforms. Tetrathionate is formed in the medium with the addition of the iodine and potassium iodide solution. Organisms containing the enzyme tetrathionate reductase will thrive in this medium. Sodium chloride supplies essential electrolytes for transport and osmotic balance.
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