Tryptose Broth is used for the cultivation of Brucella and other fastidious microorganisms from clinical samples. The media with Tryptose, and in this case with thiamine, are recommended for the isolation, cultivation and differentiation of Brucella. Sanders and Huddleson demonstrated that the addition of Dextrose and Thiamine Chlorhydrate to the media stimulates the growth of some species of Brucella. Tryptose is a source of nitrogen, vitamins and amino acids. Dextrose is the fermentable carbohydrate providing carbon and energy. Sodium chloride supplies essential electrolytes for transport and osmotic balance, and Thiamine Chlorhydrate is a growth factor. Tryptose Broth with Thiamine is also recommended for the cultivation of pathogen microorganisms for Streptococcus, Pneumococcus, Meningococcus and other fastidious bacteria.
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