BRUCELLA AGAR 500 grams/bottle

Brucella Agar, being rich in nutrients and growth factors, is very suitable to grow and isolate fastidious microorganisms. It is used to successfully isolate Brucella from diverse specimens contaminated with microflora, both saprophytes and commensals, in clinical samples as well as in foods. This medium is also used to produce clostridial toxins. It can also be utilized in the isolation of many anaerobes both of human and animal origin. Food samples can be inoculated directly on the plates of Brucella Agar, while clinical specimens are more convenient as suspensions or macerations in sterile saline solutions. Meat peptone and casein peptone provide nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is a source of vitamins, particularly of the B-group. Sodium bisulfite is the reducing agent; Sodium chloride supplies essential electrolytes for transport and osmotic balance. Dextrose is the fermentable carbohydrate providing carbon and energy. The addition of blood provides extra growth factors for fastidious microorganisms. Bacteriological agar is the solidifying agent. The addition of the Brucella Supplement (Cat. 6017) enhances the mediums selectivity for the growth of Brucella. For a better growth, Polyenrichment Supplement (Cat. 6011) and Polyenrichment CC Supplement (Cat. 6071) may be added if required. Brucella species are level 3 pathogens and cause brucellosis, a zoonotic disease. It is usually transmitted through milk, dairy products, meat and direct contact with infected animals. Note: To obtain an excellent medium for anaerobes, add 5 mg/ml of hemin and 10 µg/ml of vitamin K1 (fitomenadione) to the basal medium. Inoculate and incubate in anaerobic conditions