Trypticasein Soy Broth Without Dextrose

Trypticasein Soy Broth w/o Dextrose is a medium very rich in nutrients for general use in microbiological laboratories. The medium is used frequently in many procedures of diagnostic research or microbiology. For example, it is used for the isolation and sensitivity testing of all types of pathogens, and for the production of antigens for agglutination and serological tests. Containing two peptones as rich nitrogen sources, obtained by the enzymatic hydrolysis of casein and soy proteins, this medium supports the growth of a great variety of microorganisms, including fastidious aerobes and anaerobes. Soy peptone also contains natural sugars which promote bacterial growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance, and dipotassium phosphate is a buffering agent.